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Research Day 2011 – Anthony Eiler – Internal Validation of Yfiler for casework at the St Louis Metropolitan Police Department

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Anthony Eiler Poster

Biography: 

Anthony Eiler was born in East Ridge, TN. He completed his under graduate work at the University of Tennessee at Chattanooga where he received a biology degree with a molecular concentration. Anthony is currently a master’s student at Marshall University in the Forensic science department and is working to complete four different emphasizes DNA Analysis, Forensic Chemistry, Digital Forensics and Crime Scene Investigation.

Abstract: 

The Applied Biosystems®’s (AB) Yfiler® DNA amplification kit was internally validated at the St. Louis Metropolitan Police Department (SLMPD) for the detection of male DNA. For the internal validation of Yfiler®, sensitivity, precision, mixture, probative/non-probative, and species cross reactivity studies were performed. The Yfiler® kit was shown to be precise and sensitive when a 0.8ng amplification target was used. The mixture study demonstrated male DNA can be detected in ratios of 1:5000 parts female DNA and confirmed the kit’s ability to detect multiple male profiles. The samples used in the probative/non-probative study successfully proved the kit’s ability to amplify forensic type samples. The species cross reactivity study proved human specificity. Pending the completion of the final review and signature of the technical leader, SLMPD will implement the Yfiler® kit for forensic casework usage.

Poster:

Anthony Eiler Poster
Internal Validation of Yfiler for casework at the St Louis Metropolitan Police Department

References:

  1.  Applied Biosystems. AmpFlSTR® Yfiler™ PCR Amplification Kit. Foster City: Applied Biosystems, 2006. Print
  2. Butler, J. M. 2005. Forensic DNA typing, 2nd Edition. Elsevier Press; New York, NY. 201-222.
  3. Federal Bureau of Investigation. “Revised Validation Guidelines Scientific Working Group on DNA Analysis Methods (SWGDAM).” Federal Bureau of Investigation, July 2004. Web. Spring 2010.
  4. Gross, A. M., et al. 2008. Internal Validation of the AmpFLSTR Yfiler Amplification Kit for Use in Forensic Casework. Journal of Forensic Sciences. 53: 1.
  5. Godby, Justin. “Technical Assistance Program.” Class. Marshall University, Huntington. Spring 2010. Lecture Marshall University Forensic Science Center. 2007. Yfiler® Validation Studies. Marshall University.
  6. Mulero, J. J. et al. 2006. Development and Validation of the AmpFLSTR Yfiler PCR Amplification Kit: A male specific, Single Amplification 17 Y-STR Multiplex System. Journal of Forensic Sciences. 51: 1.

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