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Research Day 2011 – Andrew Sawin – A Comparative Analysis of Quantifiler® Duo and Plexor®HY DNA Quantification Systems

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A Comparative Analysis of Quantifiler® Duo and Plexor®HY DNA Quantification Systems.

A Comparative Analysis of Quantifiler® Duo and Plexor®HY DNA Quantification Systems

Biography:

Andrew Sawin received undergraduate training at Alma College, where he earned a Bachelor of Science in biochemistry. He is currently a second year graduate student at Marshall University where he is majoring in forensic science. He is scheduled to complete his graduate coursework on May 7, 2011, with emphases in forensic DNA analysis and crime scene investigation, and expects to begin employment in one of these fields later this year.

Abstract:

A comparative analysis of Quantifiler® Duo and Plexor®HY DNA quantification systems was conducted to assist forensic laboratories in choosing the combined human-male DNA quantification method that best meets their needs while identifying potential benefits of each system. The systems evaluated were internally validated at Marshall University Forensic Science Center prior to the initiation of this study. This study consisted of an identical sample comparison of each system’s standard curve quality metrics, sensitivity, precision, reproducibility, contamination, and mixture evaluation. Future studies include the addition of the MavenQSTTM quantification system to the comparison study as well as a study to determine the lowest concentration sample at which a Short Tandem Repeat or STR profile of equal or superior quality may be obtained.

Poster:

A Comparative Analysis of Quantifiler® Duo and Plexor®HY DNA Quantification Systems.

References:

  1. Applied Biosystems.  Quantifiler® Duo DNA Quantification Kit User’s Manual. Part Number 4391294 Rev 04/2008
  2. Barbisin, Maura, et al. Developmental Validation of the Quantifiler® Duo DNA Quantification Kit for Simultaneous Quantification of Total Human and Human Male DNA and Detection of PCR Inhibitors in Biological Samples. J Forensic Sci, March 2009, Vol. 54, No. 2
  3. Goodwin, W., et al. An Introduction to Forensic Denetics. Wiley 2007
  4. Krenke, Benjamin E., et al. Developmental Validation of a Real-Time PCR Assay for the Simultaneous Quantification of Total Human and Male DNA. Forensic Science Internation: Genetics 3 (2008) 14-21
  5. MUFSC analytical Procedures Manual. Quantitiation of DNA Extracts Using the Promega  Plexor®HY System.
  6. Promega Corporation.  Plexor®HY System for the Applied Biosystems 7500 and 7500 FAST Real-Time PCR Systems. Revised 11/07
  7. Wilson, Johnathan Price. Developmental Validation of an Improved Multiplex Assay for Use in Forensic Casework: The Simultaneous Gender-Typing and Quantification of Total Human and Male-Only DNA Within Forensic Samples. 06/08

 

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